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About High Performance Liquid Chromatographs (HPLC)

High performance liquid chromatographs (HPLC) use a liquid mobile phase to separate the components of a mixture. The components are dissolved in a solvent and forced to flow through a chromatographic column under high pressure. In the column, the mixture is resolved into its components. The components flow through a detector and a chromatogram is generated. Most high performance liquid chromatographs (HPLC) use high-pressure pumps to force solvents through packed stationary beds. Isocratic pumps use constant mobile phase composition to elute compounds. By contrast, gradient pumps vary the strength of the organic solvent to elute different compounds. Pumps with flow control are well-suited for high performance liquid chromatographs (HPLC) that deliver gradients automatically. Specifications such as flow rate, maximum pump pressure, and pump accuracy vary among devices. In simple systems, the sample is introduced with an injection valve. More complex high performance liquid chromatographs (HPLC) incorporate an autosampler with a microprocessor.   




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